Histidinol phosphate phosphatase of Mtb: structural and functional analysis
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منابع مشابه
Structural and mechanistic characterization of L-histidinol phosphate phosphatase from the polymerase and histidinol phosphatase family of proteins.
L-Histidinol phosphate phosphatase (HPP) catalyzes the hydrolysis of L-histidinol phosphate to L-histidinol and inorganic phosphate, the penultimate step in the biosynthesis of L-histidine. HPP from the polymerase and histidinol phosphatase (PHP) family of proteins possesses a trinuclear active site and a distorted (β/α)(7)-barrel protein fold. This group of enzymes is closely related to the am...
متن کاملNovel monofunctional histidinol-phosphate phosphatase of the DDDD superfamily of phosphohydrolases.
The TON_0887 gene was identified as the missing histidinol-phosphate phosphatase (HolPase) in the hyperthermophilic archaeon "Thermococcus onnurineus" NA1. The protein contained conserved motifs of the DDDD superfamily of phosphohydrolase, and the recombinantly expressed protein exhibited strong HolPase activity. In this study, we functionally assessed for the first time the monofunctional DDDD...
متن کاملSome properties of the catalytic sites of imidazoleglycerol phosphate dehydratase-histidinol phosphate phosphatase, a bifunctional enzyme from Salmonella typhimurium.
Kinetic and aggregatory properties of a partially purified preparation of D-erythro-imidazoleglycerol phosphate dehydratase-histidinol phosphate phosphatase from a derepressed mutant of Salmonella typhimurium (his012$2) have been investigated. The molecular weight in crude extracts is 300,000. Purification of the enzyme causes disaggregation, the main component keing 75,000 in molecular weight....
متن کاملStructural studies of the catalytic reaction pathway of a hyperthermophilic histidinol-phosphate aminotransferase.
In histidine biosynthesis, histidinol-phosphate aminotransferase catalyzes the transfer of the amino group from glutamate to imidazole acetol-phosphate producing 2-oxoglutarate and histidinol phosphate. In some organisms such as the hyperthermophile Thermotoga maritima, specific tyrosine and aromatic amino acid transaminases have not been identified to date, suggesting an additional role for hi...
متن کاملThe separation and partial characterization of L-histidinol phosphatase and an alkaline phosphatase of Saccharomyces cerevisiae.
Two enzymes which effected the hydrolysis of the histidine biosynthetic intermediate histidinol phosphate were isolated from Saccharomyces cerevisiae. The enzymes were separated by diethylaminoethyl Sephadex column chromatography and partially characterized. The histidinol phosphatase was shown to be a speci6c enzyme, hydrolyzing only the histidine intermediate, histidinol phosphate. It was ins...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations and Advances
سال: 2017
ISSN: 2053-2733
DOI: 10.1107/s2053273317094086